Phytate and Microbial Suspension Amendments Increased Soybean Growth and Shifted Microbial Community Structure

Phytate represents an organic pool of phosphorus in soil that requires hydrolysis by phytase enzymes produced microorganisms prior to its bioavailability plants. We tested the ability a microbial suspension made from old growth maple forest’s undisturbed mineralize phytate greenhouse trial on soybean plants inoculated or non-inoculated with suspension. MiSeq Amplicon sequencing targeting bacterial 16S rRNA gene and fungal ITS was performed assess community changes following treatments. Our results showed nodulation shoot dry weight biomass increased when applied nutrient-poor substrate mixture. Bacterial diversities root rhizosphere biotopes were relatively resilient inoculation suspension; however, structure significantly influenced. Interestingly, four arbuscular mycorrhizal fungi (AMF) identified as indicator species, including Glomus sp., Claroideoglomus etunicatum, Funneliformis mosseae unidentified AMF taxon. also observed ericoid taxon Sebacina sp. three Trichoderma spp. among species. Non-pathogenic Planctobacteria members highly dominated core hub taxa for over 80% all datasets biotopes. Overall, our study documented amendment improved plant growth.